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Top 50 Brno Startups to Watch in 2026

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Brno has established itself as a key startup location within the Czech Republic. The city is home to a diverse range of companies, with strong representation in cybersecurity and software development.

Travel technology company Kiwi.com leads the local scene with over $107M in total funding. Other notable sectors include artificial intelligence and financial technology.

Below is our list of 50 Brno startups worth keeping an eye on.

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Kiwi.com provides a travel search engine that combines flights, trains, and buses from hundreds of carriers into single itineraries. Their unique algorithm finds and connects flights from non-cooperating airlines, offering travelers cheaper and often unconventional routes.

Year Founded2012
FoundersJiri Hlavenka, Jozef Képesi, Oliver Dlouhý, Vladimir Končar
Funding Amount$107.2M
Startup SizeMajor Organization (1,001-5,000)
Last Funding StatusDebt Financing
Top InvestorsImpulse Ventures, Ondrej Tomek, Touzimsky Kapital, Jiri Hlavenka

ThreatMark provides an advanced fraud detection platform that uses AI and behavioral intelligence to secure digital transactions for businesses. Their system analyzes subtle user behaviors in real time to distinguish between legitimate customers and potential fraudsters.

Year Founded2015
FoundersKrystof Hilar, Michal Tresner, Tomáš Strýček
Funding Amount$34.6M
Startup SizeMid-Size Team (51-250)
Last Funding StatusVenture Round
Top InvestorsOctopus Ventures, Riverside Acceleration Capital, ORBIT Capital, Rockaway Ventures, EASME

Whalebone develops DNS-based cybersecurity products that protect telcos, ISPs, and large enterprises from online threats. Their security solution is designed for zero disruption, blocking malware and phishing attempts at the network level before they can reach the end user.

Year Founded2016
FoundersLubos Cerny, Richard Malovic, Robert Sefr
Funding Amount$17.6M
Startup SizeMid-Size Team (51-250)
Last Funding StatusSeries B
Top InvestorsDay One Capital, Unbound, Fazole Ventures, Martin Reitenspiess, Jan Prerovsky

Kontent.ai builds an AI-powered headless CMS that helps enterprise teams manage their content from a single unified hub. Their platform is designed to increase content velocity, reduce brand risk, and improve the overall return on investment from content.

Year Founded2015
Founders
Funding Amount€39M
Startup SizeMid-Size Team (51-250)
Last Funding StatusPrivate Equity
Top InvestorsExpedition Growth Capital

Zaitra develops artificial intelligence that runs on spacecraft to make data transmission more efficient and less costly. Their onboard software processes information directly in orbit, reducing the amount of data that needs to be sent back to Earth.

Year Founded2020
Founders
Funding Amount€1.7M
Startup SizeLean Team (11-50)
Last Funding StatusPre-Seed
Top InvestorsSunfish Partners, European Space Agency, Czech Founders VC, Technology Agency of Czech Republic, Honza Široký

Mewery creates cultivated pork grown on a unique microalgae base. This process offers a slaughter-free and sustainable alternative to traditional meat production.

Year Founded2021
Founders
Funding Amount€3.2M
Startup SizeLean Team (11-50)
Last Funding StatusGrant
Top InvestorsBig Idea Ventures, Credo Ventures, Purple Ventures, EIC Accelerator

Roger developed a financial platform to help companies overcome the challenge of long payment maturities. Their service allows businesses to get paid early on invoices, freeing up cash flow to concentrate on growth.

Year Founded2013
FoundersAdam Soukal
Funding Amount€12M
Startup SizeLean Team (11-50)
Last Funding StatusVenture Round
Top InvestorsKB SmartSolutions

ROI Hunter develops a product marketing platform that helps e-commerce companies drive more profitable growth. Their system combines performance analytics with creative automation to boost return on ad spend from social media campaigns.

Year Founded2014
FoundersKarel Schindler
Funding Amount$5.7M
Startup SizeMid-Size Team (51-250)
Last Funding StatusSeries A
Top InvestorsKAYA, Accelerasia Ventures, Agora SA

Smartlook builds a behavior analytics platform that records user sessions on websites and mobile apps to reveal why they take certain actions. The platform combines session recordings with heatmaps and event tracking to give product teams and marketers the qualitative data they need to improve their UX.

Year Founded2016
FoundersDusan Kmet, Petr Janosik, Vladimir Sandera
Funding Amount$3.9M
Startup SizeMid-Size Team (51-250)
Last Funding StatusSeries A
Top InvestorsAirbridge Equity Partners, Reflex Capital, Webnode, Novira

Casinvent Pharma develops novel drugs for treating hematological and solid malignancies. Their approach centers on inhibiting Casein Kinase 1, a protein family with a proven role in the development of various cancers.

Year Founded2020
Founders
Funding Amount€2.9M
Startup SizeFounding Team (1-10)
Last Funding StatusVenture Round
Top InvestorsDynumerics as, JIC Ventures, i&i Biotech Fund, Holecek Family Foundation, i&i Prague

40 Additional Brno Startups Worth Following

Our top ten picks are just a glimpse into the energy buzzing through Brno's startup scene. Founders are tackling challenges in diverse fields like AI, media, and healthcare, collectively raising tens of millions in funding.

Some companies are fresh on the scene, while others are well-established players in their industries. Here’s a look at the other startups that make Brno’s ecosystem one to watch.

StartupIndustryYear FoundedAmount RaisedLast Funding Round
Smartsupp logo SmartsuppAI2013$4MVenture Round
Carebot logo CarebotHealthcare2021€800kPre-Seed
Talsec logo TalsecMedia2021€1MSeed
Madfinger Games logo Madfinger GamesGaming2009€5MCorporate Round
Localazy logo LocalazyMedia2020€2.2MSeed
Lakmoos logo LakmoosData2023€510kPre-Seed
Yedem logo YedemEnvironment2019CZK13MSeed
DYNANIC logo DYNANICEnterprise2021€550kPre-Seed
Arbo Technologies logo Arbo TechnologiesCloud2021€400kSeed
DuoCards logo DuoCardsOther2000€600kVenture Round
CleverMaps logo CleverMapsAnalytics2015CZK42M
Wereldo.com logo Wereldo.comLogistics2018$1.4MSeed
StreamBee logo StreamBeeAnalytics2020€550kPre-Seed
CryptoMood logo CryptoMoodAnalytics2017$400kSeed
AI|ffinity logo AI|ffinityAI2021€420kSeed
DOT Glasses logo DOT GlassesRetail2015€1.1MPre-Seed
Flatio logo FlatioMarketplace2015€240kSeed
Eventee logo EventeeMarketing2019$500kAngel
Signi logo SigniMarketing2019€1MSeed
Pekat Vision logo Pekat VisionAI2016$750kSeed
Ashborne Games logo Ashborne GamesGaming2020CZK150MCorporate Round
USU Software logo USU SoftwareData1997
Disivo logo DisivoRetail2018CZK12.5MSeed
GREYCORTEX logo GREYCORTEXAI2016$1.3MVenture Round
Myco logo MycoBiotech2021CZK14MVenture Round
Pealock logo PealockRetail2019CZK11MSeed
Skinners logo SkinnersRetail2016CZK6MEquity Crowdfunding
Lightigo Space logo Lightigo SpaceHardware€200kGrant
Zaslat.cz logo Zaslat.czLogistics2014€800kCorporate Round
Agdata.ag logo Agdata.agSaaS2016€1.2MSeed
Oscar Senior logo Oscar SeniorHealthcare2013$30k
Able logo AbleEnterprise2011€161.8kVenture Round
Fragments logo FragmentsCrypto2018$200kPre-Seed
TagPicker logo TagPickerEnterprise2019€30kPre-Seed
Excalidraw logo ExcalidrawMedia2020
Futupilot logo FutupilotMediaCZK3MSeed
TESCAN logo TESCANMedia1991
GINA Software logo GINA SoftwareAgTech2010€120kVenture Round
Webnode logo WebnodeSaaS2008Seed
EuroStar logo EuroStarHardware1994

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